Abstract
BackgroundThe study sought to investigate the biological efficacy of methanol leave extract of Acacia auriculiformis (MEAA) via in vitro, in vivo, in silico approaches. The in vitro cytotoxicity was evaluated through brine shrimp lethality assay, and anti-inflammatory activity was determined by membrane stabilisation and protein denaturation methods (BSA and egg albumin). The in vivo antipyretic activity was examined via Brewer’s yeast induced pyrexia model.ResultsA. auriculiformis extract unveiled moderate cytotoxicity with significant anti-inflammatory efficacy (p < 0.001) compared to standard drug. This extract also exhibited dose-dependent time of paralysis and death for the worm (p < 0.001) in the anthelmintic test which was directly proportional to employed concentrations. A notable percentage of clot lysis effect (36.42 ± 1.95%, p < 0.001) was also observed for MEAA in human blood compared to control. However, this extract significantly (p < 0.05) reduced fever in a dose-dependent manner during the antipyretic experiment. Besides, in computer-aided investigations, two compounds (2,4-ditert-butylphenol and 3-hydroxy-β-damascone) revealed the best binding interaction with six proteins for cytotoxicity, inflammation, helminthic, thrombolytic and pyretic effect. Moreover, these two compounds satisfy Lipinski’s ‘Rule of Five’ and revealed drug-likeness profiles in the toxicological study.ConclusionsThese findings disclosed that methanol leaves extract of A. auriculiformis might be a potent source for anti-inflammatory, anti-helminthic, thrombolytic and antipyretic agents.
Highlights
The study sought to investigate the biological efficacy of methanol leave extract of Acacia auriculiformis (MEAA) via in vitro, in vivo, in silico approaches
Inflammation is a biological defensive response initiated by several stimuli, including irritants, chemicals, heat, infection or any immunological reactions [1]
Effect of MEAA on brine shrimp lethality bioassay To determine the cytotoxicity of MEAA, brine shrimp lethality bioassay was performed in the current study
Summary
The study sought to investigate the biological efficacy of methanol leave extract of Acacia auriculiformis (MEAA) via in vitro, in vivo, in silico approaches. Inflammation is a biological defensive response initiated by several stimuli, including irritants, chemicals, heat, infection or any immunological reactions [1]. Inflammation shows hyperthermia and pain in the living body and aims to repair damaged tissue by inactivating deleterious stimulus or pathogen. Uncontrolled acute inflammation for a prolonged period can cause drastic pain and fever in the body. These can Pyrexia is a complex immune biochemical reaction that initiates by producing various endogenous pyrogens as a response to inflammatory or infectious stimulation [4]. The presence of pathogens or tissue damage in the body activates the immune system which produces and releases pyrogenic cytokines.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
