Abstract
Objectives: The study was undertaken to evaluate the in vitro anti-inflammatory and anti-arthritic activity of the ethanolic extract of leaves of Pyrenacantha volubilis (EEPV) using human red blood cells (HRBCs) membrane stabilization and protein denaturation methods.
 Methods: In the present study, the in vitro anti-inflammatory and anti-arthritic activity of EEPV was carried out using HRBC membrane stabilization by hypotonicity-induced hemolysis and protein denaturation using egg albumin methods at various concentrations (100, 200, 400, 800, and 1000) of EEPV. Diclofenac sodium was used as reference standard.
 Results: P. volubilis was effective in inhibiting HRBC membrane stabilization and protein denaturation in a dose-dependent manner and was comparable to the standard drug diclofenac sodium.
 Conclusion: The study suggests that P. volubilis has potential anti-inflammatory and anti-arthritic activity.
Highlights
Inflammation is the body’s immune system response to any noxious stimuli and is of two types, namely, acute and chronic inflammation
Phytochemical screening Presence of camptothecin and its derivatives has been isolated from various parts of P. volubilis such as cotyledons, ripened whole fruit, and root, followed by seed coat, fruit coat, and young and mature leaves using liquid chromatography and MS analysis [2]
There was a dose-dependent increase in the percentage inhibition of hypotonicity-induced hemolysis for both extract of leaves of Pyrenacantha volubilis (EEPV) and diclofenac
Summary
Inflammation is the body’s immune system response to any noxious stimuli and is of two types, namely, acute and chronic inflammation. Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease which occurs mainly due to the formation of anti-citrullinated protein auto-antibodies. Matrix metalloproteinases (MMPs) are generated by the activation of nuclear factor kappa B (NFκB) and mitogen-activated protein kinase (MAPK) [1]. Structural rigidity of joints is destructed when MMPs along with disintegrin and thrombospondin degrade the components of extracellular matrix. Synovial proliferation takes place leading to the formation of “pannus” that sequels RA. Available nonsteroidal anti-inflammatory drugs, glucocorticoids, and disease-modifying anti-rheumatic drugs provide only symptomatic reliefs and are associated with severe side effects. Herbal medicine contains many phytoconstituents and has potential medicinal values with fewer side effects
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
