Screening Trichoderma strains isolated from soil growing Curcuma longa in Hung Yen province for treating waste after turmeric processing

Abstract

The cultivation and processing of turmeric are developing, resulting in a large amount of turmeric waste causing serious environmental pollution. Trichodermafungi are potential microbial sources for agricultural waste treatment thanks to their ability to synthesise several extracellular enzymes. In this study, 15 strains of Trichoderma were isolated from turmeric-growing soil in Hung Yen province. Among them, 4 strains, which were named HYT11, HYT12, HYT13, and HYT14, were found to have a high potential for synthesising cellulase, amylase, and protease enzymes. Based on morphological characteristics and ITS rDNA sequence analysis, 3 strains (HYT11, HYT12, and HYT13) were identified as Trichoderma asperellum and the strain HYT14 was identified as Trichoderma harzianum. All four strains showed the ability to grow and form conidia on turmeric waste. The study also determined the optimal conditions for the conidia formation of these strains, including a nutrient medium containing potato extract (200 g/l) supplemented with 2% glucose, 1% peptone, and 2% agar, and the cultivation of 7 days at 30oC. In summary, the research has selected four Trichodermastrains, HYT11, HYT12, HYT13, and HYT14, which are promising for application in treating turmeric waste.