Abstract

Anaerobic digestion can be used for the treatment of POME to reduce organic content and generate some substances, such as volatile acids, ethanol, and various gasses. During anaerobic fermentation process, microorganism requires the presence of trace elements for growth to improve their performance. This research will be carried out as a study of the presence of trace elements, such as metals (Cu2+, Mn2+, and Mg2+) that has significant effects on ethanol formation. Circulating Bed Reactor was used and operated in a batch system for 48 hours. Metal ions were screened and analyzed by using two-level factorial design method whether there is any correlation effect between the addition of Cu2+, Mn2+, and Mg2+ and ethanol formation. Several parameters which consists of Total Volatile Acids (TVA), dissolved Chemical Oxygen Demand (CODs), Volatile Suspended Solid (VSS), pH, Dissolved Oxygen (DO), and ethanol were measured every sampling. Mn metal is proven statistically affect both TVA and ethanol formation while Mg metal only affect TVA formation. Cu2+ and Mg2+ metals combination affect ethanol formation with largest detected ethanol concentration is 7,483.07 mg/L. The result from this study had identified the metal ions which has significant effect as a foundation for optimization ethanol formation.

Highlights

  • The energy used today almost entirely fossil-derived oil was known as non-renewable energy

  • Alternative energy can be obtained by using various types of substrates, such as raw materials containing saccharides, starch, dan biomass cellulose [17] that will process by anaerobic fermentation

  • Anaerobic fermentation process involves cell metabolism influenced by micronutrients, such as cofactor metal ions for enzyme catalyst eventhough the amount of it is low concentration

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Summary

Introduction

The energy used today almost entirely fossil-derived oil was known as non-renewable energy. As the production of energy from these fuels is associated to resource depletion, the identification of new and renewable sources of energy has become a matter of priority in many countries One of those alternative energy is a biofuel derived from biomass conversion. Anaerob fermentation process will produced some substances, such as ethanol and methane gas that can be alternative energy sources. Anaerobic fermentation process involves cell metabolism influenced by micronutrients, such as cofactor metal ions for enzyme catalyst eventhough the amount of it is low concentration. This research will be carried out as a study of the presence of trace element, such as metals (Cu2+, Mn2+, and Mg2+) that had the most influence on ethanol formation from palm oil mill effluent using two-way level factorial design

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