Abstract
Foeniculum vulgare Mill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer with pH 6, have been employed and antimicrobial activity tested against four reference strains. The extracted protein was subjected to 3 kDa dialysis and separation was carried out by DEAE-ion exchange chromatography and further proteins were identified by 2D gel electrophoresis. The results of Foeniculum vulgare elutes obtained from DEAE-ion exchange chromatography were tested for antibacterial activity. Elute 3 shows the highest antibacterial activity on Pseudomonas aeruginosa with a diameter of a zone of inhibition of 16 mm and IC50 value 25.02 (mcg/mL). Based on the findings of the wide usage in treatment of various ailments and day-to-day life, Foeniculum vulgare seeds were used in the present research and have shown promising antibacterial effects, which requires further proteomic research to authenticate the role of the anticipated proteins.
Highlights
Voluminous plants and plant extracts have a wide variety of resistance mechanisms to chemical, physical, and biological strains, like heavy metals, cold, drought, pathogens and contaminant attacks from bacteria, fungi, and viruses
The bacterial strains used in the study were Escherichia Coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), and Proteus vulgaris (P. vulgaris), and compared with the standard antibiotics ciprofloxacin (25 mcg/mL) and chloramphenicol (100 mcg/mL)
P. aeruginosa with a diameter of theobtained zone ofafter inhibition of 16 chromatography mm and an IC50 value of results of Foeniculum vulgare elutes ion exchange were tested for when antibacterial activity as the shown in Table
Summary
Voluminous plants and plant extracts have a wide variety of resistance mechanisms to chemical, physical, and biological strains, like heavy metals, cold, drought, pathogens and contaminant attacks from bacteria, fungi, and viruses. Numerous mutual features of plant antimicrobial peptides share with those from insects, microorganisms, and animals. They comprise characteristics, like their molecular arrangements. These features are well-signified by two plant antimicrobial peptide families, thionins and plant defensins. Foeniculum vulgare Mill., commonly called fennel, is a therapeutic plant belonging to the Umbelliferae (Apiaceae) family, is widely used in traditional medicine. This is one the most important plants, which has several pharmacological properties, both in vivo and in vitro, including anti-microbial, anti-viral, anti-inflammatory, anti-mutagenic activities, etc. In our study we adopted the gel electrophoresis technique to separate proteins by
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