Screening of susceptibility genes for bronchopulmonary dysplasia in Han premature infants

Abstract

Objective To explore the relationship between susceptibility loci polymorphism and bronchopulmonary dysplasia (BPD) in Han premature infants. Methods Twenty-eight susceptibility genes were speculated to be associated with BPD development according to the literature.And 46 cases of Han premature infants, who were hospitalized in Guangdong General Hospital from April to December 2013 and whose gestational age≤32 weeks, birth weight (BW)≤1 500 g, were prospectively selected into the study after obtaining the informed consents from the parents.Among them, 31 cases were BPD patients (BPD group), and 15 cases were non-BPD patients (control group). Blood samples were collected for the detection of 28 susceptibility genes within 3 days of hospitalization.After blood samples were extracted, and genomic DNA was amplified by PCR, the blood samples were then analyzed by single base extension technology and DNA mass spectrometry. Results Fourteen genes, whose frequencies were in Hardy-Weinberg equilibrium among the 28 susceptibility genes, were finally selected as the candidate genes for the study.Tumor necrosis factor α (TNF-α) gene rs1799724 and Toll-like receptor (TLR)-10 gene rs11096955 were founded to be possibly related to the development of BPD.A significant higher CC [93.5%(29/31 cases)]and lower TT[0(0/31 cases)] contents were presented in TNF-α gene detection in BPD group, those in the control group were 33.3%(5/15 cases), 20.0%(3/15 cases), and there was significant difference between 2 groups(χ2=18.044, P 0.05). Conclusions TNF-α gene and TLR-10 gene loci polymorphism might contribute to the development of BPD.TNF-α gene rs1799724 CC genotype and TLR-10 gene rs11096955 AC genotype might be the susceptible genotypes of BPD.TNF-α gene rs1799724 C allele carrier has a higher risk for BPD. Key words: Bronchopulmonary dysplasia; Susceptibility gene; Infant, premature; Han