Screening of Specific Genes Expressed in the Swine Tissues and Development of a Functional cDNA Chip

Chul Wook Kim,Jung Sou Yeo,Kwang Keun Cho,Byeong Woo Kim,Young Kuk Joo,Eun Jung Kwon,Kyu Tae Chang,Ki Hwa Chung,Won Yong Jung,Jung Gyu Lee,Yang Su Kang,Yeon Hee Hong

doi:10.5713/ajas.2005.933

Abstract

To develop a functional cDNA chip, specific genes expressed in the tissues of swine Kagoshima Berkshire were screened. A total of 4,434 ESTs were obtained by constructing a cDNA library from total RNA isolated from the muscle and fat tissues, affirming their functions by investigating similarity of nucleotide sequences with the database at the NCBI. Among them, 1,230 ESTs were confirmed as novel genes, which, to date, have not been identified. Attaching the genes to a cDNA microarray slide revealed expression patterns of genes in muscle and fat according to the growth stages of swine. As specific genes expressed in the muscle tissues of swine with body weight of 30 kg, 60 genes including actin, myosin, tropomysin, transfer RNA-trp synthetase, Kel-like protein 23, KIAA0182 and COI, Foocen-m, etc were obtained. In addition, 18 novel genes were obtained. As specific genes expressed in fat tissues of swine with body weight of 30 kg, 47 genes including annexin II, Collagen, Fibronectin, Pleckstrin homology domain, serine protease, etc were obtained. 21 novel genes were also obtained. The genes specifically expressed in the muscle and fat tissues of swine affect contraction and relaxation of the muscle and the fat. However, studies on the expression mechanisms of the genes are insufficient. To reveal species of structural genes in swine muscle and fat tissue, interrelation studies in expression and function of genes by using the cDNA chip should be conducted. (Asian-Aust. J. Anim. Sci. 2005. Vol 18, No. 7 : 933-941)

Highlights

Chul Wook Kim*, a, Kyu Tae Chang1, a, Yeon Hee Hong2, a, Eun Jung Kwon, Won Yong Jung, Kwang Keun Cho Ki Hwa Chung, Byeong Woo Kim3, Jung Gyu Lee3, Jung Sou Yeo4, Yang Su Kang5 and Young Kuk Joo6
In markers S0064, S0066, S0102 or TNF, located on particular, the genetic traits associated with meat quality of chromosome 7, which are associated with back fat thickness, Berkshires have not yet been studied sufficiently by reason sirloin cross-section, meat quality traits, and boar taint (Jung et al, 1989; Bidanel et al, 1996), respectively, have
Heart-fatty acid binding protein (H-FABP) gene affecting IMF (Gerbens et al, 1997) and the relationship between IMF and microsatellite SW1823S0003 (74-79 cM) region existing on chromosome 6 has been investigated (Grindflek et al, 2001)

Summary

INTRODUCTION

Swine leucocytes antigen (SLA) complex exists studies on meat quality or assays of genes associated with on chromosome 7 (Geffrotin et al, 1984) and microsatellite the meat quality of Berkshires have been carried out. In markers S0064, S0066, S0102 or TNF, located on particular, the genetic traits associated with meat quality of chromosome 7, which are associated with back fat thickness, Berkshires have not yet been studied sufficiently by reason sirloin cross-section, meat quality traits, and boar taint (Jung et al, 1989; Bidanel et al, 1996), respectively, have. For the last several years, great efforts have been made to develop a genetic map containing unknown gene markers and well-known genes in connection with meat quality traits. CDNA microarray technology has been applied to simultaneous expression of numerous genes and discovery of genes in a large scale, as well as genetic polymorphism screening and mapping of chromosomal DNA clones. We further attempt to apply the cDNA chip in establishing high quality seed-swine and evaluating meat quality according to varieties of swine and tissues of pork

MATERIALS AND METHODS

RESULTS

DISCUSSION