Abstract

The communication system in bacteria is mediated by chemical signal molecules in a process that is known as quorum sensing. Inactivation of this process, called quorum quenching (QQ), could be a potential strategy for controlling plant pathogenic bacteria. This study aimed to identify QQ bacteria as biocontrol agents against Pectobacterium carotovorum subsp. carotovorum (Pcc). For this purpose, the ability of obtained isolates from the rhizosphere to degrade acyl-homoserine lactone signalling molecules was investigated in the presence of gamma-caprolactone. In vitro and GC-MS analyses confirmed the consumption of GCL by some isolates which identified as species of Pseudomonas, Bacillus and Erwinia based on their phenotypic characteristics and partial 16S rRNA gene sequences and, Pseudomonas rhizosphaerae was introduced as QQ agent. Also, in vivo evaluation, Bacillus pumilus, Pseudomonas fluorescens and Pseudomonas sp. reduced soft rot in potato tubers by 98% as compared to the control inoculated with Pcc.

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