Screening of proteolytic bacteria from tauco Surabaya based on pathogenicity and selectivity of its protease on milky fish (Chanos chanos) scales for healthy and halal collagen production

Abstract

This study was reported our work on the advanced screening of proteolytic bacteria from tauco Surabaya based on pathogenicity of the isolates and the selectivity of its proteases on milky fish (Chanos chanos) scales. Determination of pathogenicity was carried out through hemolytic test on blood agar medium. Formation of clear zones around the growing bacterial colonies indicate the pathogenic bacteria, whereas no clear zones around the bacterial colonies are formed in the results of non-pathogenic bacteria test. Protease which has selectivity to milky fish scales can hydrolyse peptide bonds in milk fish scales to produce soluble collagen that can be measured as dissolved protein. The results showed that the order of pathogenicity qualitatively from the lowest to the highest were the isolate of HTcUM7.1<HTcUM6.1.1<HTcUM2<HTcUM10< HTcUM9.1<HTcUM6.2.2< HTcUM8. Crude extract of protease from HTcUM7.1, HTcUMi0 and HTcUM6.2.2 successively have specific activity of protease were 6.044±3.390, 0.311±1.099 and 1.718±0.040 U/mg. Furthermore, it was able to produce the dissolve protein with concentration as much as 0.1015±0.040, 0.0930±0.044 and 0.0825±0.004 mg/mL. Overall results showed that HTcUM7.1 was the most potential proteolytic bacteria as source of protease for production halal and healthy collagen from milky fish scale.