SCREENING OF PEAS (PISUM SATIVUM) VARIETIES/ LINES AGAINST FUSARIUM WILT (FUSARIUM OXYSPORUM .SP.PICI) AND IN VITRO EVALUATION OF FUNGICIDES AGAINST MYCELIAL GROWTH OF PATHOGEN

Abstract

Twenty Peas (Pisum sativum L.) varieties/ lines were evaluated against Fusarium wilt caused by Fusarium oxysporum .Sp.pici by sowing them in sick plot during the year of 2016-17 at the Plant Pathology Research Institute, Faisalabad. Each cultivar/line was planted in a single row of three meter length, with plant to plant and row to row distances of 15cm and 30 cm respectively and replicated thrice by following Randomized Complete Block Design (RCBD). Out of these twenty varieties/ lines 13 including check variety Olympia were found highly susceptible ranging from 53.2 to 83.5% plant mortality. Six varieties/lines were susceptible ranging from 30.3 to 44.1 % plant mortality. Only a single variety Garrow performed as moderately resistant by showing 21% plant mortality in the field. Efficacy of five fungicides against Fusarium oxysporum .Sp.pici, at various concentrations was evaluated in-vitro and significant variations among treatments was observed. In general there was a significant decrease in mycelial growth of the fungus with an increase in concentration of fungicides. Tilt (Propiconazol),( Daconil (Chlorothalonil) and Crest (Carbendazim) were the most effective fungicides in inhibiting the growth of the fungus in descending order. The Tilt almost 90% inhibited the growth @ 50µg/ml concentration, Daconil and Crest exhibited intermediate effectiveness. Topsin-M (Thiophanate-methyl) and Score (Difenoconazole) were the least effective fungicides.Ahmad, M. A., S. M. Iqbal, N. Ayub, Y. Ahmad and A. Akram. 2010. Identification of resistant sources in chickpea against Fusarium wilt. Pak. J. Bot, 42: 417-426.Borum, D. E. and J. Sinclair. 1968. Evidence for systemic protection against Rhizoctonia solani with vitavax in cotton seedlings. Phytopathology, 58: 976-&.Davies, D., G. Berry, M. Heath and T. Dawkins. 1985. Pea (Pisum sativum L.). Grain Legume Crops. Collins, London, UK: 147-198.Food and Agriculture Organization of the United Nations. 2011Hagedorn, D. 1984. Compendium of pea diseases. 57 p. Am. Phytopathol. Soc., St. Paul, Minnesota, USA.Hannan, A., S. T. Sahi, I. Ahmed and A. A. Choudhry. 2014. Differential impact of Fusarium oxysporum f. sp. pisi on resistance source of pea genotypes and its chemical management. Pakistan Journal of Phytopathology, 26: 91-96.Haware, M. P. 1978. Eradication of Fusarium oxysporum f. sp. ciceri Transmitted in Chickpea Seed. Phytopathology, 68: 1364.Haware, M.P. and Nene, Y.L., 1982. Races of Fusarium oxysporum f.sp. ciceri. Plant disease, 66 (9), pp.809-810.Hulse, J. H. 1994. Nature, composition, and utilization of food legumes. Expanding the Production and Use of Cool Season Food Legumes. Springer Netherlands, pp. 77-97.Ilyas, M., M. Iqbal and K. Iftikhar. 1992. Evaluation of some fungicides against Fusarium oxysporum f. sp. ciceris and chickpea wilt. Pakistan Journal of Phaytopahtology, 4: 5-8.Iqbal, S. M. 2005. Screening of chickpea genotypes for resistance against Fusarium wilt. Mycopath (Pakistan).Javaid, I. A., A. Ghafoorm and R. Anwar. 2002. Evaluation of local and exotic pea Pisum sativum germplasm for vegetable and dry grain straits. Pak. J. Bot, 34: 419-427.Khan, I., S. Alam and A. Jabbar. 2002. Selection for resistance to Fusarium wilt and its relationship with phenols in chickpea.Khan, S. A., A. Awais, N. Javed, K. Javaid, A. Moosa, I. U. Haq, N. A. Khan, M. U. Chattha and A. Safdar. 2016. Screening of pea germplasm against Fusarium oxysporum f. sp. pisi and invitro management through chemicals. Pakistan Journal of Phytopathology, 28: 127-131.Khokhar, M. 2014. Production status of major vegetables in Pakistan, their problems and suggestions. Agric. Corner, 9.Kraft, J. M. 1994. Fusarium wilt of peas (a review). Agronomie, 14: 561-567.Maitlo, S., R. Syed, M. Rustamani, R. Khuhro and A. Lodhi. 2014. Comparative efficacy of different fungicides against fusarium wilt of chickpea (Cicer arietinum L.). Pakistan Journal of Botany, 46: 2305-2312.McPhee, K. 2003. Dry pea production and breeding. Food, Agri Environ, 1: 64-69.Nawab, N. N., G. M. Subhani, K. Mahmood, Q. Shakil and A. Saeed. 2008. Genetic variability, correlation and path analysis studies in garden pea (Pisum sativum L.). J. Agric. Res, 46: 333-340.Nene, Y., M. Haware and M. Reddy. 1981. Chickpea diseases: resistance-screening techniques.Pande, S., J. N. Rao and M. Sharma. 2007. Establishment of the Chickpea Wilt Pathogen Fusarium oxysporum f. sp. ciceris in the Soil through Seed Transmission. The Plant Pathology Journal, 23: 3-6.Persson, L., L. Bødker and M. Larsson-Wikström. 1997. Prevalence and pathogenicity of foot and root rot pathogens of pea in Southern Scandinavia. Plant Disease, 81: 171-174.Steel, R. G. D. and J. H. Torrie. 1980. Principles and procedures of statistics, a biometrical approach. McGraw-Hill Kogakusha, Ltd.Sundar, A. R., N. Das and D. Krishnaveni. 1995. In-vitro antagonism of Trichoderma spp. against two fungal pathogens of Castor. Indian Journal of Plant Protection, 23: 152-155.Vyas, S. C. 1984. Systemic fungicides. Systemic fungicides.