Screening of Fusarium moniliforme as Potential Fungus for Integrated Biodelignification and Consolidated Bioprocessing of Napier Grass for Bioethanol Production

Abstract

A fungus capable of producing ethanol from various carbon substrates was screened for direct ethanol production from lignocellulose. Fusarium moniliforme BIOTECH 3170 produced ethanol from glucose, xylose, and cellobiose after three days with theoretical yields of 86.4%, 68.6%, and 45.4%, respectively. The coculture of glucose and xylose progressed sequentially at 79.2% of the theoretical yield, with both sugars completely consumed in five days. The solid-state consolidated bioprocessing of cellulose produced 25.2 g/L of ethanol after 20 days. After 28 days of the integrated biodelignification and consolidated bioprocessing of Napier grass at solid-state conditions, up to 10.5 g/L of ethanol was produced, corresponding to an ethanol yield of 0.032 g/g biomass. Given a sufficient carbon source, the screened fungus could produce up to 42.06 g/L ethanol. F. moniliforme BIOTECH 3170 demonstrated the characteristics of a fungus for potential ethanol production from cellulose, mixed sugars, and lignocellulosic materials.