Abstract
The cost of bioethanol production from lignocellulosic materials is relatively high because the additional processes of delignification and saccharification are required. Consolidated bioprocessing (CBP) simultaneously uses the multiple processes of delignification, saccharification, and fermentation in a single reactor and has the potential to solve the problem of cost. Some wood-degrading basidiomycetes have lignin- and cellulose-degrading abilities as well as ethanol fermentation ability. The white rot fungus Schizophyllum commune NBRC 4928 was selected as a strong fermenter from a previous study. The lignin-degrading fungus Bjerkandera adusta and polysaccharide-degrading fungus Fomitopsis palustris were respectively added to S. commune ethanol fermentations to help degrade lignocellulosic materials. Bjerkandera adusta produced more ligninase under aerobic conditions, so a switching aeration condition was adopted. The mixed culture of S. commune and B. adusta promoted direct ethanol production from cedar wood. Fomitopsis palustris produced enzymes that released glucose from both carboxymethylcellulose and microcrystalline cellulose. The mixed culture of S. commune and F. palustris did not enhance ethanol production from cedar. The combination of S. commune and cellulase significantly increased the rate of ethanol production. The results suggest that CBP for ethanol production from cellulosic material can be achieved by using multiple fungi in one reactor.
Highlights
In bioethanol production using lignocellulosic material derived from non-food biomass, delignification and saccharification are required [1,2]
Bjerkandera adusta is reported to produce manganese peroxidases (MnPs) and other lignin-degrading in a single step and the fungi for degrading cedar wood and fermenting ethanol (Sc/Ba or Sc /Fp) enzymes [29,30]
Bjerkandera adusta IWA5b used in the present study was isolated in our laboratory were inoculated into the reactor flask at the same time
Summary
In bioethanol production using lignocellulosic material derived from non-food biomass, delignification and saccharification are required [1,2]. These pretreatments of biomass are performed by physical methods such as steam explosion [3,4] or by chemical methods such as acid [5,6,7,8] or alkaline hydrolysis [9,10]. These methods produce substances that inhibit ethanol fermentation by yeast [11,12]. The concept of consolidated bioprocessing (CBP) that simultaneously uses the multiple processes of delignification, saccharification, and fermentation in a single reactor has been
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