Screening of Enrofloxacin and Ciprofloxacin Residues in Chicken Meat by High-Performance Liquid Chromatography

A H Ahmad,Disha Pant,M K Verma,Nidhi Arya,Parul Rawat,Sonam Sharma

doi:10.9734/jpri/2020/v32i2130753

Abstract

Enrofloxacin and Ciprofloxacin antibiotics are widely used in chicken production for prophylaxis and therapeutics purposes. Existence of these antibiotic residues in chicken meat can pose hazards to human health. The present study was aimed to assess the residue level of these antibiotics in chicken meat. Chicken meat samples (including muscle, liver, kidney and fat) from poultry farms and retail market were collected. High Performance Liquid Chromatography (HPLC) was used for screening of enrofloxacin and ciprofloxacin residues in chicken meat samples. The analysis revealed that 43.58% meat samples were positive for enrofloxacin and 38.71% for ciprofloxacin residues. Out of it, 45.17% samples were having concentration above the MRL for enrofloxacin and 50.28% for ciprofloxacin. So it can be concluded that the usage of these antimicrobial in chicken lead contamination of meat and it may cause resistance in consumers and seems to be a public health threat.

Highlights

2.3 Standards and Calibration CurvesEnrofloxacin and its metabolite ciprofloxacin is belongs from fluoroquinolone class
The present study was aimed to assess the residue levels of these antibiotics in chicken meat and compare with the permissible Maximum Residue Limits (MRL) in different districts of Uttarakhand
The analysis revealed that 43.58% meat samples were positive for enrofloxacin and 38.71% for ciprofloxacin residues

Summary

Introduction

2.3 Standards and Calibration CurvesEnrofloxacin and its metabolite ciprofloxacin is belongs from fluoroquinolone class. Enrofloxacin is a potent inhibitor of bacterial DNA Topoisomerase II (Gyrase) and the DNA Topoisomerase IV which are essential enzymes of cellular processes including DNA replication [2]. These antibiotics have a broad spectrum and high efficacy against pathogens. In India, due to extra label use of enrofloxacin for prophylactic and therapeutic purposes, drug residue may be found in poultry meat. Consumption of such meat may act as a potential public health hazard. By using a powerful separation technique, such as HPLC, coupled with a UV detector and reverse phase column

Methods

Results

Conclusion