Screening of Bioactive Compounds of Ricinus communis Using GC-MS and FTIR and Evaluation of its Antibacterial and Antifungal Activity

Abstract

The objectives of this study were detection of the secondary metabolite products and evaluation antibacterial and antifungal activity. Bioactives are chemical compounds often referred to as secondary metabolites. Sixteen bioactive compounds were identified in the methanolic extract of Ricinus communis. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of Ricinus communis revealed the existence of the1, 2, 3, 4-Butanetetrol, [S-(R*, R*)]-, Ribitol, 3-Ethoxy-1, 2-propanediol, DL-Arabinose, p-Dioxane-2, 3-diol, D-Limonene, Dodecanoic acid, 3-hydroxy-, Methyl 6-oxoheptanoate, Dithiocarbamate, S-methyl-, N-(2-methyl-3-oxobutyl)-, (5β)Pregnane-3, 20β-diol, 14α, 18α-[4-methyl-3-oxo-(1-oxa-4-, 3-(N, N-Dimethllaurylammonio)propanesulfonate, Cetene, Gibberellic acid, Geranyl isovalerate, Phenol, 4-(1, 1, 3, 3-tetramethylbutyl)-, Picrotoxinin, and α-N-Normethadol. The FTIR analysis of Ricinus communis leaves proved the presence of alkanes, and alkyl halide, Amine, Aldehyde, and Alkane which shows major peaks at 781.17, 875.68, 923.90, 1018.41, 1240.23, 1319.31, 1361.74, 1361.74, 2357.01, 2850.79 and 2920.23. Clinical pathogens were selected for antibacterial activity namely, Pseudomonas eurogenosa, Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, and Proteus mirabili. Ricinus communis has maximum zone against Klebsiella pneumonia (5.000±0.19)