English

Abstract

The aims of this study were the analysis of the secondary metabolites and evaluation of the antibacterial and antifungal activity of Alternaria alternata . Twenty six bioactive compounds were identified in methanolic extract of Alternaria alternata . The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of A. alternata revealed the existence of the α-acetyl L-serine, 2(5H)-furanone, 6-oxabicyclo[ 3.1.0]hexan-3-one, D-glucose,6-O-α-D GALACTOPYRANOSYL, DL-arabinose, ƹ-N-fommyl-Llysine, 2-[4-(2-hydroxyethyl) 1-piperazineethanesulfonic acid) (HEPES), thrietol, 2-O-heptyl, 2-deoxy-2- fluoro-1,6-anhydro s-d-glucopyranose, d-ribo-hexos-3-ulose, Α-D-glucopyranoside, O-α-Dglucopyranosyl-( 1.fwdarw.3)-s-D-fru, maltose, 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl, desulphosinigrin, uric acid, midazole-4-carboxylic acid, 2-fluoro-1-methoxymethyl-ethyl ester, geranyl isovalerate, 1-nitro-s-d-arabinofuranose, tetraacetate, glycyl-D-asparagine, α-D-xylofuranose, cyclic 1,2:3,5-bis(butylboronate), estra -1,3,5(10)-trien-17s-ol, glucobrassicin, N-2,4-Dnp-L-arginine, dasycarpidan-1-methanol, acetate(ester) and 5alpha androstane-3,17-monooxime. The fourier transform infrared (FTIR) analysis of A. alternata proved the presence of aromatic rings, aliphatic fluoro compounds, tetiary amine, C-N stretch, ammonium ions, organic nitrate, methylene-CH. asym, and normal polymeric O-H stretch which shows major peaks at 711.73, 846.57, 873.75, 1026.13, 1149.57, 1205.51, 1238.30, 1409.96, 1631.78, 2517.10, 2854.65, 2924.09, 3059.75 and 3271.27. A. alternate had maximum zone formation (5.04 ± 0.29) mm against Klebsiella pneumonia . Keywords: Alternaria alternata , bioactive compounds, gas chromatography mass spectrometry (GCMS), fourier transform infrared (FTIR)