Screening of Antibiotype among Environmental Isolates of Acinetobacter spp. in Hospital Setting

Abstract

Cross infection through hospital environment has been a major challenge to control nosocomial infection. This has been worse with the emergence of multidrug resistant strains. The present study was conducted to study the pattern of antibiotic resistant group of Acinetobacter spp. in hospital environment to understand possibility of nosocomial infection. The study was conducted for a period of six months during which Acinetobacter spp. from 269 samples of hospital environment (air sample, surface swab and hand swab from healthcare workers) were identified by conventional microbiological method and antibiogram was performed by Kirby-Bauer disc diffusion method and NCCLS guidelines. Bacterial isolates obtained from both samples were tested for their relatedness based on their resistivity pattern among the tested antibiotics. Of the total environmental samples 212 samples were found to be positive and a total of 183 gram negative isolates were obtained. Of the total gram negative isolates, 84.2% (154/183) Acinetobacter spp. were isolated. Analysis of MDR isolates revealed 70.8% (109/154) Acinetobacter spp. which was MDR. A total of 12 different antibiogram patterns were found with five different antibiotic groups tested. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 203-208 DOI: http://dx.doi.org/10.3126/njst.v13i2.7737