A Study on Surgical Site Infection, their Bacteriological Profile and Antimicrobial Susceptibility Pattern in Tertiary Care Hospital, Rajkot, Gujrat

Abstract

Introduction: Surgical site infection is defined as infection occurring within 30 days after surgical operation and affecting either incision or deep tissues at the operation site. SSIs cause substantial morbidity and mortality. This study was aimed at obtaining the incidence of SSI and to evaluate the risk factors as well as to formulate an antibiotic policy. Aims and Objectives: To determine the incidence, risk factors and the bacteriological profile for SSI and their antimicrobial susceptibility patterns at PDUMC, Rajkot. Materials & Method: Total of 872 pus samples from surgical ward were studied from January 2022 to June 2022. The isolates were identified by colony morphology, Gram’s stain and conventional biochemical reactions. Antibiotic sensitivity testing was done by Kirby-Bauer disc diffusion method as per CLSI guidelines 2021. Results: Total 272 microbial pathogens were isolated. Out of these, 28(10.29%) were Gram- positive Cocci, 244(89.70%) were Gram-negative Bacilli whereas 64 showed polymicrobial growth. Out of total Gram negative isolates, 108(44.26%) showed Klebsiella species followed by Pesudomonas aeruginosa 72(26.50%). Out of total Gram positive isolates, Staphylococcus aureus 16(57.14%) is the predominant pathogen followed by Coagulase Negative Staphylococci 12(42.85 %). Conclusion: The study gives an insight into bacterial pathogens and their antibiotic sensitivity patterns isolated from surgical site infections. Surveillance of SSI helps in early detection, appropriate use of antibiotics & to reduce the rate of SSI.