Screening of Antibacterial Activity of Azadirachta Indica (Neem) by Using Leaf Extract against Escherichia Coli and Bacillus Subtilis

Abstract

Azadirachta Indica (Neem) is the member of the Meliaceae family. It is rich source of Antibacterial properties and Antioxidants. They have consist 65 biochemically active compounds. In ancient time plant and plant product are used for medicine. Neem (Azadirachta indica) is the most useful and traditional medicine used in India. They are also used in cosmetics and beauty aid. The demand of neem products are increasing day by day. In agricultural sector is now becoming a major consumer of the neem products. They also have use agricultural, Pesticides made from neem are product if natural plant origin. They are biodegradable and non-toxic. They do not have any side effects. It is widely used in Ayurvedic, Unani and Homeopathic. All the Parts of neem plants like Neem leaves, Fruits, Seeds, Organic Product, Roots and Bark are utilized to make various medicine. But Neem leaves and its constituents have been shown to display many properties like- Antiulcer, Antimalarial, Antifungal, Antibacterial, Antiviral, Antimutagenic and Anticarcinogenic. The present study was evaluate Antimicrobial activity of Neem extract against some pathogens. The Antibacterial effect of Azadirachta Indica against Gram Negative Bacteria (Escherichia coli) and Gram Positive Bacteria (Bacillus Subtilis) was determined using the MIC (Minimum Inhibitory Concentration) on Agar Media by Disk Diffusion Method. The inhibition zone were measured in millimetre with the help of Zone Reader. The data show that the inhibition zone of Neem Extract were greater than each of Phytoconstituents 80mg/ml concentration was the more effective than 40 mg/ml and 20mg/ml was more effective than 10mg/ml.