Abstract
Essential oils are great sources of nature .Today they are used a lot in food industry. One of these essential oils is Fumitory oil. The effects of growth inhibition of Fumitory oil on gram positive bacteria(Staphylococcus areous and Bacillus cereous), gram negative bacteria(E.coli and Salmonella typhymorium) and fungi(Candida albicans and Aspergillus flavus) were studied in invitro. The disc diffusion method was used to evaluate the zone of bacterial and fungal growth inhibition of the oil. Minimal Inhibitory Concentration (MIC) of the oil was used as broth dilution method for bacteria and fungi .Minimal bacteriocidal Concentration (MBC) and Minimal Fungicidal Concentration (MFC) of the oil were determined by agar surface culture method . Tetracyclin and nistatin were used as positive control and normal saline was used as negative control. Zone of inhibition in bacteria and fungi were not significant. In E.coli and Salmonella typhymorium, Minimal Inhibitory Concentration(MIC) was 62.5?li/ ml and Minimal Fungicidal Concentration(MFC) of the oil was 125 µml/lit.On Staphylococcus areous, Minimal Inhibitory Concentration(MIC) was 15.9?li/ ml and Minimal Fungicidal Concentration(MFC) of the oil was 62.9 µml/lit.The oil didn’t inhibited of fungi growth.The Fumitory oil was found to be bacteriostate and bacteriocide about some bacteria but it didn’t had any significant antifungal effect.
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