Screening of Amorpha fruticosa and Ailanthus altissima extracts for genotoxicity/antigenotoxicity, mutagenicity/antimutagenicity and carcinogenicity/anticarcinogenicity

Abstract

The aim of the present study was to evaluate the potential genotoxic/antigenotoxic, mutagenic/antimutagenic, and carcinogenic/anticarcinogenic effect of Amorpha fruticosa (AF) fruit, Ailanthus altissima bark hexane (AAEH) and methanol (AAEM) extracts on a model system Saccharomyces cerevisiae. Plants were identified and extracted by Ekaterina Kozuharova. Three concentrations of each extract were tested – 10, 100 and 1000 µg/ml. In vitro pro-oxidant/antioxidant activities were evaluated by DPPH and DNA topology assay. The potential genotoxic/antigenotoxic, mutagenic/antimutagenic and carcinogenic/anticarcinogenic effects were revealed in vivo by: Zimmermman’s test on Saccharomyces cerevisiae diploid strain D7ts1, and Ty1 retrotransposition test on S. cerevisiae haploid strain 551. Zeocin was used as a positive control. Based on the in vitro antioxidant activity the extracts could be arranged as follows: AF>AAEM>AAEH. AAEH possessed moderate oxidative potential. No genotoxic and mutagenic capacity was obtained in vivo for extracts tested. The levels of total aberrants, convertants and revertants were comparable with the control ones. No Ty1 retrotransposition was induced by extracts treatment. Further, the extracts possessed well-expressed antigenotoxic, antimutagenic and anticarcinogenic activity. Significant reduction of the total aberrants, reverse point mutations and Ty1 retrotransposition was obtained. Only the AF extract was found to reduce the levels of zeocin-induced mitotic gene conversion. The three extracts did not possess any genotoxic, mutagenic and carcinogenic effect on Saccharomyces cerevisiae. Based on their protective activity, they can be arranged as follows: AF>AAEM>AAEH which corresponds well with their phytochemical composition. Further experiments could provide more detailed information concerning the mode of action of extracts, as well as their main constituents.