SCREENING OF AEGIALITIS ROTUNDIFOLIA ROXB. LEAVES FOR THEIR ANTIOXIDANT POTENTIAL, POLYPHENOL CONTENT AND FLAVONOID CONTENT

Abstract

The present research is focused on investigating the phytochemical analysis and antioxidant activity of Aegialitis rotundifolia Roxb. leaf. Different solvent extracts of A. rotundifolia Roxb. leaves are screened for secondary metabolites by qualitative and quantitative analysis. The resultant phytochemical screening proves that the plant leaves consist of various secondary metabolites. The quantitative analysis proves that phenolic compounds and flavonoids are dominant among them. Four different antioxidant activity methods, namely, ABTS radical scavenging activity, phosphomolybdate assay, peroxide activity and FRAP (Ferric Reducing Antioxidant Power), are used for assessing the activity of the leaf extract. Ethanolic and aqueous extracts of the leaf have high potency of antioxidant activity and reported dose-dependent activity. Similar promising antioxidant results are also reported for ABTS radical scavenging activity (82.4% reduction at 200 µg mL-1 of ethanol and 90.0% reduction at 200 µg mL-1 of aqueous extract), phosphomolybdate assay (82.4% reduction at 200 µg mL-1 of ethanol and 74.5 % reduction at 200 µg mL-1 of aqueous extract) and peroxide activity (92.1 % reduction at 200 µg mL-1 of ethanol and 93.1 % reduction at 200 µg mL-1 of aqueous extract). The antioxidant activity reported with ethanol (157.4 mmol Fe2+/g) and aqueous extract (129.8 mmol Fe2+/g) was also studied by analyzing with FRAP reduction. The obtained findings confirmed the high antioxidant activity of plant leaf extract and its application for pharmacological activity.