Abstract
This study screened lactic acid bacteria (LAB) in pickle and cured meat, explored their potential biocontrol ability against Penicillium digitatum on citrus fruit, and evaluated the inhibitory substances produced by promising biocontrol agents. A total of 224 strains were isolated. Thirteen isolates demonstrated clear inhibition zones to P. digitatum and they were identified to be Lactobacillus plantarum, Lactobacillus parafarraginis, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus buchneri, and Weissella paramesenteroides. Washed cell suspension and cell-free supernatant (CFS) of 13 isolates inhibited the growth of P. digitatum in vitro. L. plantarum CKXP13 and L. plantarum CWXP24 showed biocontrol activity in vivo. Antifungal activity of CFS of L. plantarum CKXP13 and L. plantarum CWXP24 was significantly reduced after pH-neutralization, catalase, protease treatment. It suggested that the antimicrobial substances produced by L. plantarum CKXP13 and L. plantarum CWXP24 included organic acid, hydrogen peroxide and proteinaceous compound. Mining of genome data from L. plantarum CKXP13 and L. plantarum CWXP24 showed the presence of bacteriocin encoding genes that 5 bacteriocin encoding genes were identified in L. plantarum CKXP13 and 1 bacteriocin encoding genes in L. plantarum CWXP24. These results suggested that L. plantarum CKXP13 and L. plantarum CWXP24 have potential as biocontrol agents of citrus green mold.
Published Version
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