Abstract
In order to improve phosphorus utilization in calcareous soil, a fungi Z60 with strong phosphate-solubilizing activity was isolated from crop rhizosphere of Shanxi calcareous soil by tablet separation. According to its morphological properties and ITS rDNA sequence, its position in phylogenetic development tree was defined, then dissolving characteristics and acid production were further studied. The strain was identified as Aspergillus sp., with maximum activity of phosphatedissolving reaching 714.4, 624.1, 305.2, 108.3 mg L-1respectively in tricalium phosphate, aluminum phosphate, ferric phosphate and phosphate rock liquid culture. For different C and N sources sucrose and sodium nitrate were optimum for Z60, with the C source sequence as mannitose glucose mannitose lactose starch; for N source the order was potassium nitrate ammonium nitrate ammonium chloride ammonium sulfate sodium nitrate. HPLC analysis showed that Z60 produced oxalic acid in all kinds of N source, formic acid in ammonium nitrogen as N source, lactic acid in ammonium nitrate as N source, and tartaric acid in ammonium chloride or potassium nitrate as N source. Z60 has a strong ability to dissolve hard-tosolve phosphate, therefore is a promising microbial resource of high efficiency biological phosphorus fertilizer for calcareous soil.
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