Screening for anti-inflammatory and peripheral analgesic activity of Coleus amboinicus leaves using wistar albino rats

Abstract

We have abundant plant resources with many health benefits. Use of plant material for health benefits will help in reduce the cost of drugs and also provide a substitute drug that is less expensive. Coleus amboinicus is plant largely distributed in Dakshina kannada and is used for its medicinal properties as household cure. We planned to find out the anti-inflammatory and peripheral analgesic activity of Aqueous Extract of leaves of Coleus amboinicus. Wistar albino rats were randomly selected and tests were performed by dividing the animals into three groups- control, standard, test.Aqueous extract of leaves of Coleus amboinicus was prepared using soxhlet apparatus. The dose of leaf extract used was 500mg/kg. Data was analysed by applying one way ANOVA followed by Dunnets multiple comparison test using Graphpad Instat.The results obtained were statistically significant. In Carragenan induced inflammation model significant inhibition of inflammation was shown by the test drug at the end of 3 hrs and in Acetic acid induced writing test significant inhibition of occurrence of writhes was noted by the test drug.This study validates the folkloric use of aqueous extract of leaves of Coleus amboinicus (AECA) at the dose of 500mg/kg for its anti-inflammatory and peripheral analgesic activity.