Abstract
<p>On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (<em>Achatina fulica</em>). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate (µ) 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).</p><p><strong>How to Cite</strong></p><p>Wijanarka, W., Kusdiyantini, E. &amp; Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (<em>Achatina fulica</em>) and Test the Ability of Cellulase Activity. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 386-392. </p>
Highlights
One of agricultural pests is Snail (Achatina fulica) or golden snail
Snail (Achatina fulica ) or Golden snail (Pomacea canaliculata) population can be controlled by presenting a predator, that is Solenopsis geminata
The foremost aim of this study was to found celulolityc bacteria in the digestive tract Snail (Achatina fulica) and quantify cellulase activities from them, prompting us to search for a rapid and inexpensive alternative enzyme that fulfilled our need for a suitable assay for fusion of protoplast
Summary
One of agricultural pests is Snail (Achatina fulica) or golden snail. Snail (Achatina fulica ) or Golden snail (Pomacea canaliculata) population can be controlled by presenting a predator, that is Solenopsis geminata. Control of snail is supposed to get rid of it, and processes it into something that produces benefits. Bacteria in the digestive tract of golden snail are reported to have a cellulolitic activity. These bacteria can produce cellulase enzymes to degrade cellulose to glucose. As time, increasing the need of fuel began to be addressed by producing bioethanol, which is one of the renewable fuel source and can be made in a way that is relatively quick when compared with petroleum fuels from non-renewable. Bioethanol has great prospects for this life began to develop ways of production of materials variety
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