Abstract
<p>A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 <sup>0</sup>C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. <em>Bacillus </em>sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24<sup>th</sup>, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (<em>Hermetia illucens</em>) have competencein organic waste degradation, because in Black Soldier Flays<em> Larvae’s</em> gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially <em>Bacillus </em>sp,</p><p><strong>How to Cite</strong></p><p>Supriyatna, A., &amp; Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (<em>Hermetia illucens</em>) Feeding with Rice Straw. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 314-320. </p>
Highlights
METHODSRice straw as organic waste has a chemical composition about32.1% cellulose, 24% hemicellulose and 18% lignin (Howard et al, 2003) Results of other studies Wannapeera et al (2008) showed that rice straw containing 35.7% hemicellulose, 32% cellulose, lignin 22.3%, and 10.1% ash
Feeding hay to the larvae allows the bacteria non-normal intestinal bacteria come into the intestine Black Soldier fly larvae
The benefits of this research is, it is known that the larvae of the black soldier has the potential to be used to degrade organic waste in the gut because the larvae are cellulolytic bacteria that aids in the hydrolysis of cellulose
Summary
Rice straw as organic waste has a chemical composition (in dry weight) about32.1% cellulose, 24% hemicellulose and 18% lignin (Howard et al, 2003) Results of other studies Wannapeera et al (2008) showed that rice straw containing 35.7% hemicellulose, 32% cellulose, lignin 22.3%, and 10.1% ash. Larvae Black Soldier has been found to contain Bacillus subtilis microbes on the skin and intestines (Yu et al, 2011). B. subtilis produce lipase enzymes that function down fats into fatty acids and glycerol (Pouderoyen et al, 2001; Ma et al, 2006; Singh et al, 2010). The benefits of this research is, it is known that the larvae of the black soldier has the potential to be used to degrade organic waste in the gut because the larvae are cellulolytic bacteria that aids in the hydrolysis of cellulose. A total of 25 ml inoculum was inserted to 250 ml medium of cellulose enzymes production (PES) and incubated with a speed of 1500 rpm at a temperature of 35 0C for 48 hours (liquid culture), the suspension was stored at 40C. To stop the enzyme activity, added DNS, 1.5 ml, and heated as long as 5 minutes, the enzyme activity was measured using a spectrophotometer at a wavelength of 540 nm (Miller, 1959)
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