Abstract
Objective To screen and verify the expression profiles of circular RNAs (circRNAs) in gastric cancer (GC) with genetic risk. Methods Microarray analysis was performed on 5 pairs of GC tissues to measure the expression levels of circRNAs and to investigate the expression differences of circRNAs in GC, and hierarchical clustering used to screen out the differently expressed circRNAs. In order to verify the reliability of the chip, 4 abnormally expressed circRNAs were randomly selected, and detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) in 36 pairs of gastric cancer tissues and their matched normal tissues. Results As compared with the normal tissues, the expression of 1.5 times higher than those in the gastric cancer tissues was determined as the differential expression. It was found that 44 of them were up-regulated in circRNAs and down regulated in 56 circRNAs. The results of 4 circRNAs (circRNA_0004789, circRNA_002373, circRNA_0004104 and circRNA_0005615) showed that there was no significant difference between RT-PCR detection and chip detection (circRNA_0004789: t=1.134, P=0.374; circRNA_0023736: t=2.308, P=0.147; circRNA_0004104: t=0.795, P=0.510; circRNA_0005615: t=3.157, P=0.087, respectively). Conclusion Some of the circRNAs are abnormally expressed in tissues with a genetic risk of GC, which are likely to be the key risk factors for GC. Key words: Gastric carcinoma; Heredity; Circular RNAs; Microchip
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call