SCN5A (G892A) gene is associated with AV block conduction disorder in north Indian population

Abstract

Objective- Electrical abnormalities of the heart cause the cardiac arrhythmia with or without essential structural heart disease. Due to genetic variation, cardiac arrhythmia can occur in any age group patients as well as in healthy people. The present study aimed to examine the allele and genotype of SCN5A (G892A) gene in cardiac conduction disorder (especially AV block conduction disorder) cases and comparison with healthy controls. Method- A total number of 104 cases and 104 controls were enrolled in this study. DNA was extracted using salting out method followed by polymerase chain reaction amplification and restriction endonuclease digestion (using AluI restriction enzyme). Digested PCR products were identified using agarose gel electrophoresis and stained with ethidium bromide. Results- There was a strong association of SCN5A G892A polymorphisms (GA vs. GG, OR= 4.01 and AA vs. GG OR= 3.12) with cardiac conduction disorder. Further multivariate logistic regression analysis, after adjustment for age, gender showed that when compared with wild type GG genotype, carriers of the A alleles had an increase risk of cardiac conduction disorder (OR-1.89, 95%CI- 0.78-1.67, p value- <0.0001). Conclusion- In conclusion the results of the present study suggest that the SCN5A G892A gene polymorphism carries an increased risk for cardiac conduction disorder in north Indian populations.