Abstract
The intracellular binding site of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] was determined via preliminary biochemical analysis of radioactive 1,25(OH)2D3 association with various chick tissues and then by direct autoradiographic visualization. When vitamin D-deficient chicks were injected intracardially with physiological doses of tritiated 1,25(OH)2D3 and killed 2 h later, 2–3 times more radioactivity was found in the intestinal mucosa than was present in equal weights of pancreas, parathyroid, or liver tissue. Very little tritium was found in muscle tissue. The intestinally localized radioactivity was predominantly associated with the nuclear chromatin fraction, and binding of 1,25(OH)2[3H]D3 to the nucleus was maximal 2 h after injection and at a dose of at least 0.52 nmol. Using this dose and time period, autoradiographic studies were done on duodenum and thoracic muscle of rachitic chicks injected with radioactive 1,25(OH)2D3 (11.2 Ci/mol). Thin sections of tissue were prepared for thaw and dry mount s...
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