Scientific Standardization of Different Stem extracts of Cissus quadrangularis

Abstract

Background Perennial herb known as Cissus quadrangularis is found across the tropical world and has medicinal effects. It is one among the plants that is most regularly used as medicine in India.Aim The present study was aimed to study the scientific standardization of different stem extracts of Cissus quadrangularis.Methodology The determination of the physicochemical constants was done using powdered shade dried Cissus quadrangularis Linn. stem material. Aqueous chloroform ethanol and pet ether were used to create the solvent extracts.Results The preliminary phytochemical screening showed presence of carbohydrates proteins amino acids glycosides like saponin and flavonoids tannins phenols alkaloids and steroids. On physiological evaluation loss of drying was found to be 6.4 WW extractive value of pet ether extract was found to be 3.6 WW chloroform extract 4.7 WW ethanol extract 7.2 WW and water 8.9 WW. The total flavonoid content of pet ether extract was found to be 19.2537plusmn0.0632 microgmL chloroform extract 33.7294plusmn0.0574 microgmL ethanol extract 59.8601plusmn0.0363 microgmL water extract 45.6305plusmn0.0735 microgmL. The total phenolic content of pet ether extract was found to be 19.867plusmn0.0565 microgmL chloroform extract 19.669plusmn0.0654 microgmL ethanol extract 404plusmn0.0199 microgmL and water extract 25.658plusmn0.0343 microgmL. TLC of pet ether extract showed constituents having retention factor Rf values chloroform extract showed constituents having Rf values 0.55 0.46 ethanol extract showed constituents having Rf values 0.815 and 0.584 and water extract showed constituents having Rf values 0.234.Conclusion In the current study Cissus quadrangularis plants were examined for pharmacognostic characterization physiochemical parameter measurement phytochemical screening and TLC examinations of the crude extracts