Scientific Assessment Of The Anti-Inflammatory And Wound Healing Potential Of Campanula Lyrata Subsp. Lyrata, A Turkish Folk Remedy

Abstract

The aim of the present study is to evaluate the anti-inflammatory and wound healing activities of the extracts prepared from the aerial parts of Campanula lyrata Lam. subsp. lyrata (Campanulaceae) by using in vivo methods in order to confirm the traditional utilization. n-Hexane, diethyl ether, ethyl acetate (EtOAc), methanol (MeOH) and aqueous extracts were separately prepared from the air-dried and powdered plant materials. Carrageenan-, and serotonin- induced hind paw edema, 12-Otetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema and acetic acid-induced increase in capillary permeability models were employed in mice for the anti-inflammatory activity assessment. Wound-healing activity was investigated by using incision and excision wound models along with hydroxyproline determination and histopathological analyses. MeOH extract displayed significant antiinflammatory effect in the carrageenan- and serotonin- induced hind paw edema model and in acetic acidinduced increase in capillary permeability model with the values of 25.3, 27.8 and 31.8%, respectively. MeOH extract was also found to have significant wound healing potential in the incision and excision wound models with the values of 26.9 and 39.6%, respectively. MeOH extract ointment treated group tissues also showed enhaced hydroxyproline content. The present study confirms the anti-inflammatory