Abstract
Chlorophyll pigmented grains were extracted from leaves of palm trees and maintained in aqueous solutions containing an organic solvent. Their transmittance spectra were measured for visible wavelengths, from 400 to 700 nm. Four dissolutions were prepared to consider different pigment concentrations. The transmittance measurements show a somewhat spectrally structured band between 474 and 650 nm, limited by the light extinction (scattering + absorption) due to the pigments.
Highlights
Characterization of biological tissues in terms of how much they scatter and absorb electromagnetic radiation has been an issue of great relevance for many decades
Many theoretical approaches have been devised to describe the propagation of electromagnetic radiation through biological tissues, including those based on Monte Carlo simulations [6] and radiative transfer models [7,8]
There are certain biological tissues from which some of their components have been obtained since long time to be analyzed due to the key role they have in the photosynthesis process [9]
Summary
Characterization of biological tissues in terms of how much they scatter and absorb electromagnetic radiation has been an issue of great relevance for many decades. Visible relative transmittance spectra [Trl(λ) with λ as the wavelength of the incident light] are obtained using a standard spectrophotometer From these measurements, a direct inversion based on Lambert law [10] allows to obtain the attenuation or extinction coefficient μext = μext(λ) from inversion of Trl(λ) = exp (-μextb) with b as the distance travelled by the light through the pigmented medium. If some of the pigments have conserved, after the extraction process, their granular form with submicron sized dimensions, the extinction coefficient is the sum of scattering plus absorption (μext = μsca + μabs). In this regime of extinction, if the scattering units act independently, the extinction coefficient is proportional to the volume fraction f occupied by the pigments in the solu-
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