Abstract
Three-dimensional (3D) cell cultivation systems in vitro are promising biomodels to study the metabolic processes of healthy and diseased human heart cells. Cardiac 3D-spheroids are mimicking cell living conditions and, therefore, they can be employed to investigate processes that occur in both extra-cellular and intra-cellular environments. In this study, human healthy and dilated left ventricle myocardium-derived mesenchymal stem cells (hmMSCs) were grown for 5 days in two different cell cultivation systems in vitro: (i) attached to the cell culture flask (2D-cultivation) and (ii) in spheres (3D-cultivation). Then 2D- and 3D-cultivated healthy and pathological hmMSCs have been investigated by generation-collection mode of scanning electrochemical microscopy (GC-SECM) using 2-methylnaphthalene-1,4-dione (menadione or MD) as a redox mediator. Healthy 2D-cultivated hmMSCs were more redox active, therefore, faster responded to MD and were more sensitive to the high MD concentrations compared to the pathological hmMSCs. 3D-cultivation of pathological cells improved their redox activity and made healthy hmMSCs more resistant to the MD. Data of this study show that SECM measurements, using redox mediator MD, can be applied to investigate changes of intra-cellular redox status of human healthy and dilated hmMSCs grown under different conditions. Moreover, the intra-cellular redox status of dilated hmMSCs can be purposefully improved by 3D-cultivation with further their application for therapeutic purposes.
Published Version
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