SAT665 Effects Of Weight Cycling On Hepatic Protein Carbonylation And Lipid Peroxidation

Abstract

Abstract Disclosure: M.A. Djalali-Gomez: None. J.A. Bogart: None. S. Duran Ortiz: None. D.E. Berryman: None. J.J. Kopchick: None. E.O. List: None. Over two-thirds of the U.S. population is overweight or obese which has been shown to increase oxidative stress. Oxidative stress damages biological molecules such as lipids, proteins, and DNA, ultimately contributing to the development of numerous diseases including cancer and diabetes. Increasing awareness of the health issues caused by obesity leads many to attempt weight loss. Unfortunately, successful weight loss is usually temporary as an estimated 80% of individuals who lose weight will gain it back within a year. Repeated cycles of weight gain and weight loss are known as weight cycling. While the prevalence of obesity and weight cycling has increased in recent years, research on the long-term health consequences of weight cycling is limited. In the current study, we evaluated how diet stability (i.e., weight-cycled diets vs stable diets) and immediate diet (i.e., diet at the time of tissue collection; high fat vs low fat) affect oxidative stress by measuring hepatic lipid peroxidation and protein carbonylation from mice exposed to long term weight cycling or a stable diet regimen with high or low-fat diets. Our results indicate that the immediate diet significantly impacted hepatic oxidative stress, as mice on a high-fat diet prior to sacrifice had significantly greater levels of hepatic protein carbonylation (p=.020) as well as hepatic lipid peroxidation (p=.010) compared to mice on a low-fat diet. Importantly, our results also indicate that diet stability significantly impacted hepatic oxidative stress as weight cycled mice had significantly greater levels of hepatic protein carbonylation (p=.030) and hepatic lipid peroxidation (p=.008) compared to mice fed stable diets. Overall, our results indicate that weight cycling, independent of immediate diet, increases oxidative damage in the livers of mice compared with mice fed stable (non-cycled) diet regimens. Presentation: Saturday, June 17, 2023