Sarcoptes scabiei dermatitis in adult sheep: an immunohistochemical study of 34 chronic cases with extensive lesions

Abstract

Ovine sarcoptic mange is a contagious ectoparasitic skin disease, seen in many countries with sheep production. Although several studies concerning dermatopathology have been published, the local cutaneous immune response to Sarcoptes scabiei has not been studied by immunohistochemistry. The present study aims to evaluate immunohistochemically the adaptive cellular immune response in chronic natural cases with extensive gross lesions. Facial and foot skin biopsies of 32 ewes and 2 rams were obtained, and moreover from the scrotal scabietic lesions of the 2 rams. Each biopsy was bisected and processed for paraffin and cryostat sections. Mites were not observed in the vast majority of skin histology sections. Epidermal hyperplasia and chronic inflammation were the main histopathologic features. The dermal inflammatory infiltrate was mixed, dominated by eosinophils and lymphocytes equally. Tissue sections immunostained with a panel of monoclonal antibodies showed among lymphocytes an almost exclusively T-cell population (CD3+), while CD79a + cells were sparse. T-helper cells (CD4+) were predominant versus T-cytotoxic cells (CD8+) in 4:1 to 5:1 ratios. The mixed inflammatory infiltrate combined with the immunohistochemical findings suggest both a type-I and type-IV hypersensitivity reactions during the chronic course of the disease. Moreover, all these chronic cases in adult sheep are recorded into the hypersensitivity form of sarcoptic mange (“classical or ordinary” scabies) and no cases of the hyperkeratotic form of the disease (“Norwegian or crusted” scabies) were found.