Salt requirements for membrane transport and solute retention in some moderate halophiles

Abstract

Many species of bacteria isolated from saline environments require Na + specifically for membrane transport. Transport occurs by a Na + symport process energized by an electrochemical gradient of Na + ions. The gradient at neutral pH appears to be produced by a primary electrogenic extrusion of protons coupled to a secondary, outwardly directed Na + pump, a Na +/proton antiporter. At alkaline pH Vibrio alginolyticus may also produce the gradient by an energy-dependent primary extrusion of Na + ions. Alteromonas haloplanktis and Vibrio costicola require salts in the medium to retain intracellular solutes. For A. haloplanktis the effects of the salts are primarily osmotic. For V. costicola, only NaCl is effective in retaining solutes and Na + is required by this organism to maintain the membrane potential. In Escherichia coli a single substitution in the nucleotide sequence of the gene coding for the melibiose transport protein changed the cation specificity of the transport system. The possible ecological significance of this finding has been considered.