Abstract
For almost a decade, the number of Salmonella enterica subspecies enterica serovar Infantis-positive broiler flocks has been steadily increasing in Slovenia, doubling the number of positive holdings in only a few years. Since multidrug resistant S. Infantis isolates are highly prevalent in the broiler meat industry and may represent a public health concern through the food chain, we aimed to investigate the antimicrobial susceptibility, genetic diversity, and biofilm-forming ability of S. Infantis from Slovenian broiler flocks. A total of 87 S. Infantis strains isolated from broiler faeces in the period between 2007 and 2013 were studied. The samples originated from 41 farms which were subcontractors of three major food business operators and from two autonomously operating holdings (farms). Isolates were phenotypically tested for their susceptibility to 14 antimicrobials from nine classes by determining the minimum inhibitory concentration with the microdilution method. Only 8% of the isolates were susceptible to all of the antimicrobial agents tested, while 88.5% of the isolates were multidrug resistant, with the most common resistance pattern CipNxSSuT (65.5%) followed by CipNxSuT (17.2%). Pulsed-field gel electrophoresis (PFGE) divided the strains into five clusters (A-E) comprising 16 distinct XbaI PFGE profiles. Sixty-five out of 87 isolates were grouped in clusters A and B, with the predominant PFGE profiles A1 and B1 encompassing 33 and 28 isolates, respectively. A vast majority of the isolates (75/87) showed >90% PFGE profile similarity. The biofilm-forming capacity of the tested isolates, determined with crystal violet assay in polystyrene microwell plates, was generally weak. The average biofilm formation for persistent strains was higher than for presumably nonpersistent strains; however, the difference was not significant. It seems that S. Infantis persistence on broiler farms is more related to its widespread occurrence in the broiler production chain and ineffective disinfection protocols than to its ability to form biofilm.
Highlights
The presence of Salmonella in poultry is considered to be a risk factor for the contamination of meat and eggs
In order to prevent zoonotic transmission of Salmonella, national control programmes for Salmonella in poultry are set in the European Union (EU) to reduce the prevalence of certain serovars [1]
Most of the EU Member States met their Salmonella reduction targets for poultry, and Salmonella prevalence is declining in these animal populations
Summary
The presence of Salmonella in poultry is considered to be a risk factor for the contamination of meat and eggs. In order to prevent zoonotic transmission of Salmonella, national control programmes for Salmonella in poultry are set in the European Union (EU) to reduce the prevalence of certain serovars [1]. Most of the EU Member States met their Salmonella reduction targets for poultry, and Salmonella prevalence is declining in these animal populations. A significant declining trend of human salmonellosis cases was observed between 2008 and 2012 [2]. Salmonella still ranks first among the causative agents of food-borne outbreaks in the EU. The two most commonly reported Salmonella serovars in humans in 2017 were S.
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