Abstract
Aims: To identify and estimate salivary VEGF of breast cancer patients using non invasive method. Methods: ELISA, Zymography, Immunoblot. Results: We observed that saliva of breast cancer patients express appreciably high VEGF compare to non breast cancer saliva samples. Conclusion: The findings indicate salivary VEGF as a potential breast cancer marker using non invasive method.
Highlights
Angiogenesis is essential for the growth of most primary tumors
The salivary MMP-2 activity of breast cancer patient and non breast cancer patient was developed by zymography
4.3 Immunoblot of Salivary Vascular endothelial growth factor (VEGF), MMP-2 and MMP-9 Immunoblot of salivary proteins clearly shows that MMP-2 and MMP-9 are activated in breast cancer saliva (Fig 3)
Summary
Angiogenesis is essential for the growth of most primary tumors. Tumors can absorb sufficient nutrients and oxygen by simple diffusion up to a size of 1–2 mm, at which point their further growth requires the elaboration of a vascular supply. VEGFs are predominantly produced by endothelial, hematopoietic, and stromal cells in response to hypoxia and upon stimulation by growth factors such as transforming growth factor beta (TGFβ), interleukins, or platelet-derived growth factors (PDGFs) VEGF A, which is often referred to as VEGF ( in general ), has been studied more than the other members of this family and it has several distinct isoforms (VEGF121, VEGF145, VEGF148, VEGF165, VEGF183, VEGF189 and VEGF206) because of alternative splicing of mRNA from a single, 8-exon VEGFA gene (chromosome 6p) (Ferrara & Alitalo, 1999; Carmeliet, 2002) During angiogenesis, it increases migration and mitosis of endothelial cells, matrix metalloproteinase activity, αvβ activity, creation of blood vessel lumen. When the cell becomes hypoxic, HIF1α persists and the HIF1α/β complex stimulates VEGF release
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