Abstract
The ongoing Ebola outbreak in West Africa has resulted in fast-track development of vaccine candidates. We tested a vesicular stomatitis virus vector expressing Ebola virus glycoprotein for safety in pigs. Inoculation did not cause disease and vaccine virus shedding was minimal, which indicated that the vaccine virus does not pose a risk of dissemination in pigs.
Highlights
The ongoing Ebola outbreak in West Africa has resulted in fast-track development of vaccine candidates
Our data indicate that, rVSV∆G/EBOVGP can replicate in pigs, this vaccine virus does not result in overt clinical disease, and virus shedding is minimal
Because a high dose of the vaccine was directly injected intradermally into the snouts of the animals in this study and yet did not cause disease, it is unlikely that vaccination of humans with the rVSV∆G/EBOVGP vector would result in a productive infection with clinical disease in domestic pigs during a spillover event
Summary
The ongoing Ebola outbreak in West Africa has resulted in fast-track development of vaccine candidates. One of these vaccine candidates is based on a recombinant vesiculovirus vector, species vesicular stomatitis Indiana virus (here designated and more commonly known as VSV) expressing the EBOV strain Mayinga glycoprotein (here designated rVSV∆G/ EBOVGP; formerly designated VSV∆G/ZEBOVGP) [2,3,4]. To evaluate the safety of rVSV∆G/EBOVGP in a relevant livestock species, we inoculated pigs with this vaccine and compared clinical signs and virus replication with those of a recombinant wild-type VSV vector (rVSVwt) described previously [3].
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