Saccharomyces cerevisiae Ghosts Using the Sponge-Like Re-Reduced Protocol

Abstract

Sponge-Like protocol for Ghosts preparation showing an increasing interest while it exceed the range of the E lysis gene protocol for Bacterial Ghosts preparation which is restricted only to gram-negative bacteria. The protocol can be used for nearly all bacterial strains. In this study, it succeeded for the first time to produce ghosts from Eukaryotic cell. In this study, S. cerevisiae yeast was turned to Ghost cells using Sponge-Like protocol for Ghosts preparation. However, centrifugation step was eliminated to avoid self-adhering or shrinking of the yeast empty cells. Instead, decantation was used. One unique properties of the yeast is that, it is able to decant. The protocol for ghost cells preparation was also reduced to use only the Minimum Inhibitory Concentration (MIC) of each of NaOH, SDS, NaHCO 3 and H 2 O 2 . NaHCO 3 was used instead of CaCO 3 due to the variation in the cell wall between the prokaryotic (Bacteria) and the eukaryotic (yeast) cells. Light and Scanning electron microscope were used to evaluate the quality of the S. cerevisiae Ghosts (S.c.Gs). Spectrophotometer was used to evaluate the amount of the realized DNA and Protein. The study show successful yeast ghost preparation with correct 3D structure.