S107 Effects of Exposure to Cigarette Smoke Condensate on Pneumococcal Gene Expression in Relation to Biofilm Formation

Abstract

Although cigarette smoking is well-recognised as being the strongest independent risk factor for development of invasive pneumococcal disease, little is known about its direct effects on the expression of virulence factors by the pneumococcus. The primary objectives of the current study were to investigate the effects on gene expression in relation to biofilm formation following exposure of the pneumococcus to cigarette smoke condensate (CSC). Strain 172 (serotype 2–3F) of the pneumococcus was exposed to CSC (20–160 µg/ml) for 16 hours at 37°C in 6-well tissue culture plates to facilitate adherence and biofilm formation. Following incubation, biofilm associated with the adherent bacteria was stained with 0.1% crystal violet, extracted and assayed spectrophotometrically. In the case of gene expression, the bacteria (2 x 10 8 colony forming units/ml) were exposed to CSC (160 µg/ml) or solvent for 60 min at 37°C, after which RNA was extracted and converted to cDNA by reverse transcriptase-PCR (RT-PCR) and whole genome gene expression profiles determined using the Streptococcus pneumoniae TIGR4 DNA Microarray Chip. Six microarrays were performed (in triplicate for the control and CSC-treated systems). Exposure of the pneumococcus to CSC resulted in dose-related augmentation of biofilm formation which attained statistical significance ( P hk11 (histidine kinase) and its cognate response regulator, rr11 , which has been implicated in biofilm formation by S. mutans . Relative to the non-exposed control system, the respective levels of up-regulation of each gene were 19.7- and 22.5-fold ( P P