Abstract
Fifty five isolates of bacteria associated with maize were tested for the production of IAA using Fe-HClO4 and Fe-H2SO4 reagents. The production of IAA in the absence and the presence of 100 mg/L of trp was in the range of 0.24 to 9.99 and 0.14 to 12.51 mg/L, respectively. There were 16 isolates showing a remarkable decrease in the production of IAA in the presence of trp. Twelve isolates were further assayed for siderophore production on the CAS agar medium according to the method modified by Srivastava et al. (2013) but there was only one of them showing the ability. The length of the changed color edges starting from the borderline between the two media PS and CAS produced by isolate DDN10b changed from 1.30±0.10 cm at 2 DAI to 2.67±0.21 cm at 4 DAI. Effects of 5 potential isolates on plant growth of maize were studied under pots experiments. All isolates demonstrated an increase in shoot length, fresh shoot and root weight, and dry mass of one month old plants compared to the control. The best three were VTN2b (Bacillus subtilis), DDN10b (Burkholderia sp.) and TDB1 (Bacillus sp.) showing effects on dry mass equivalent to 50% NPK treatment or 75% NPK treatment and were suggested for field trials.
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