Abstract

This study evaluated the development of Bacillus amyloliquefaciens in high salinity conditions and effects of adding this bacteria strain into the culture medium of Artemia franciscana. In the first experiment, B. amyloliquefaciens was inoculated at a density of 106 CFU/mL in different salinities of 15‰ (control treatment), 80‰, 85‰, 90‰, 95‰ and 100‰. After 10 days, the result showed that B. amyloliquefaciens could be able to grow and develop at the salinity as high as 90‰ (3.02 ±0.01 log CFU/mL). The second experiment determined the ability of B. amyloliquefaciens to improve environmental factors and bottom sediment in Artemia culture condition at the salinity of 90‰, in the presence or absence of muddy sediment. Artemia were cultured at the density of 100 ind./L and were fed with shrimp feed (Number 0 nursing feed). After 15 days, the survival rate (74.8%) and the mating rate (83.3%) in treatment supplemented with B. amyloliquefaciens, and without muddy sediment were statistically different from those treatments without bacteria supplementation. In the treatment with B. amyloliquefaciens and muddy sediment, Artemia reached higher length (8.7mm) and higher fecundity compared to others (p

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