S phase kinase-associated protein 1 can inhibit the proliferation and invasion of intrahepatic cholangiocarcinoma

Abstract

Objective To investigate the expression of S-phase kinase-associated protein 1 (SKP1) in intrahepatic cholangiocarcinoma and to investigate its effects on proliferation, migration and invasion of intrahepatic cholangiocarcinoma. Methods Western blotting assay was used to determine the expression difference of SKP1 in intrahepatic cholangiocarcinoma and normal tissues. The SKP1 interference vector and empty vector were constructed and stably transfected into intrahepatic cholangiocarcinoma cells by lentivirus packaging to form an experimental group and a control group. The expression of SKP1 was detected by immunoblotting assay. The proliferation of experimental group and control group after transfection was detected by cell counting kit-8 (CCK-8) assay. The migration ability was detected by scratch test. The invasion ability of Transwell was detected by Transwellassay. Results The expression of SKP1 in intrahepatic cholangiocarcinoma was (1.701±0.215) times, and the difference was statistically significant (t=8.512, P<0.05). Lentiviral transfection of RBE cells decreased the expression of SKP1, and the Western blotting assay decreased the experimental group by (3.632±0.335) times, and the difference was statistically significant (t=11.376, P<0.01). The CCK-8 experiment showed that the relative survival rates of the experimental group at 24, 48, and 72 hours were (53.136±0.314)%, (42.661±0.248)% and (35.037±0.203)%, respectively, and the differences were statistically significant (t=8.594, 8.899, 9.210, P<0.01). The scratch test showed that the number of migrated cells in the experimental group at 24 and 48 hours (41.198±3.092) and (64.773±6.437) were significantly lower than those in the control group (113.487±3.514) and (168.767±8.151), and the difference was statistically significant (t=5.430, 6.224, P<0.05). The Transwell experiment showed that the number of perforated cells in the experimental group was (34.185±3.124), which was significantly lower than that in the control group (121.234±6.814), and the difference was statistically significant (t=9.918, P<0.05). Conclusion The expression of SKP1 in intrahepatic cholangiocarcinoma is higher than that in normal tissues. Decreasing SKP1 expression can inhibit the proliferation, migration and invasion of intrahepatic cholangiocarcinoma. Key words: S phase kinase associated protein-1; Intrahepatic cholangiocarcinoma; Ubiquitination; Proliferation; Migrate; Invasion