Effects of SKP2 expression on radio-sensitivity of esophageal carcinoma

Abstract

Objective To explore the correlation of S-phase kinase-associated protein 2 (SKP2) expression with radio-sensitivity of esophageal carcinoma.Methods Detecting the level of SKP2 in four different esophageal carcinoma cell lines using Western-blotting.Constructing p-pcDNATM 3.1/myc-His A-SKP2 exprcssion and SKP2 RNAi vector and transfecting SKP2 low or high expression cell,respectively.Clone forming assay was used to detect the cell proliferation ability of different groups after 5 Gy γ-ray irradiation.Results The sequence of SKP2 expression level in four different esophageal carcinoma cell line was KYSES10＞KYSE450＞EC9706＞KYSE150.Compared with parent control cells,the clone forming ability of SKP2 high expression cells was significantly increased (F=3.53,P＜0.01 ),showing high SKP2 expression depression the radio-sensitivity of KYSE 150 cell.In contrast,the clone forming ability of KYSE510 cell was significantly decreased after SKP2 knock-down (F=5.23,P＜0.05),showing low SKP2 expression promoting the radio-sensitivity of KYSE510 cell.Conclusion SKP2 expression was correlated with radio-sensitivity of esophageal carcinoma,but further study was needed to explore the molecular mechanism. Key words: Esophageal neoplasms; S-phaes kinase-associated proteins; RNA interference; Radiation tolerance