Abstract

Abstract Four groups of individually caged hens (14-16 hens per group) were used in each of 3 trials. The cages were suspended over concrete channels lm wide × 18 cm deep. When manure beneath the hens reached a depth of ca. 2.5cm it was treated once with an even distribution of a 0.5% granular formulation of S-31183 at rates of 0, 20, 40, and 80mg AI/m2. At the end of Trials 1 and 2 (14 days) all manure in the channels was removed prior to the subsequent trial. Manure was sampled on days 3 or 4, 7, and 14 in Trials 1 and 2, and on days 4, 7, 14, 21, 28, 35, and 42 in Trial 3 and either frozen for later bioassays or immediately placed into plastic wash basins (28cm diam, 8cm deep) and held under screen emergence cones for 3 wk for adult fly emergence. For the bioassays, 4 156g paper souffle cups for each collection date were filled with manure and 25 one-day-old house fly larvae seeded into each cup. The cups were placed over sand in larger cardboard cups and held at ca. 27°C and 50% RH until the larvae pupated. Pupae were removed, counted, placed in plastic vials, and held for adults to eclose. At the end of 14 days (Trials 1 and 2) the manure was removed and the treatments were repeated.

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