Abstract
Gastric Cancer, is widespread despite medical science progress, and is characterized by a low rate of survival and poor recovery of patients throughout the world. Hence, single nucleotide polymorphisms associated with the binding status of miRNA in the target gene that affects gene expression level in this cancer. The purpose of this study was to investigate the association of rs12977 polymorphism alleles in RAC1 and the prevalence of gastric cancer as well as its progress concerning other clinical and pathological features of Iranian patients.A computational study was initially performed to explore the signaling pathways that correlated with the activity of hsa-miR-3605-3p by way of linking rs12977 was located at its seeding region responsible for the binding with hsa-miR-3605-3p. Real-time PCR-HRM analysis on genomic DNA of stomach cancer (no: 140) and control (no: 54) samples, genotyping approach was carried out to determine the association of rs12977 with the incidence/progress of gastric cancer.Results indicated a significant correlation between polymorphism and primary tumor, the degree of progression of cancer and metastasis (p < 0.05). In addition, rs12977 of the RAC1 gene can consider as a potential biomarker for demonstrating the progression and spread of gastric cancer.
Published Version
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