Abstract
Carotenoid synthesis inEscherichia coli,when transformed with plasmid containing a carotenoid gene cluster fromErwinia herbicola(pPL376), is regulated by RpoS. When the plasmid was transformed intoE. colimutants that wereoxyRminus, the intracellular carotenoid concentration dramatically increased from that observed in anoxyRplus allele. The higher carotenoid concentration in these mutants correlated with an increase inrpoStranscription as indicated by β-galactosidase activity from arpoS::lacZpromoter fusion. This indication of a higher concentration of carotenoids correlated with an increased resistance to hydrogen peroxide and near-ultraviolet radiation (310–400 nm; near-UV).
Published Version
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