Abstract
BackgroundCancer metastasis is the major reason for cancer related deaths, and the mechanism of cancer metastasis still unclear. RPLP1, a member of a group of proteins known as ribosomal proteins, is associated with tumorigenesis and primary cell immortalization and is involved in cellular transformation. However, the expression and potential function of RPLP1 in TNBC remain unclear.MethodsThe expression of RPLP1 in TNBC tissues and cell lines were detected with Real-Time PCR and western blotting. 81 cases of TNBC tissue samples and adjacent non-tumor tissue samples were tested by immunochemistry to determine the correlation between the RPLP1 expression and clinicopathological characteristics. In vitro, we determined the role and mechanistic pathways of RPLP1 in tumor metastasis in TNBC cell lines.ResultsIn this study, we detected high levels of RPLP1 expression in TNBC samples and cell lines. RPLP1 is upregulated in triple-negative breast cancer (TNBC) tissues and cells, and high expression levels correlate with an increased risk of recurrence and metastasis. Furthermore, high RPLP1 expression was associated with a poor prognosis and was an independent prognostic marker for TNBC. In RPLP1-induced cancer metastasis, RPLP1 may increase cancer cell invasion, which is likely the result of its effect on the cancer cell epithelial-mesenchymal transition.ConclusionsAltogether, our findings indicate RPLP1 is a poor prognostic potential biomarker and anti-metastasis candidate therapeutic target in triple-negative breast cancer.
Highlights
Cancer metastasis is the major reason for cancer related deaths, and the mechanism of cancer metastasis still unclear
The expression of RP Large P1 (RPLP1) in Triple-negative breast cancer (TNBC) tumors, adjacent normal tissue, and cell lines To confirm the results of our prior proteomics data, we used immunohistochemistry staining to profile the expression of RPLP1 in paraffin embedded TNBC tissues
We demonstrated that RPLP1 expression is low in the adjacent normal tissues, while it is high in TNBC tissues
Summary
Cancer metastasis is the major reason for cancer related deaths, and the mechanism of cancer metastasis still unclear. Triple-negative breast cancer (TNBC), which is defined by a lack of the estrogen receptor (ER), progesterone receptor (PR), and the human epidermal growth factor receptor 2 (HER2) receptor, is resistant to conventional hormone and Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit [8]. Together these subunits are composed of four RNA species and approximately 80 structurally distinct ribosomal proteins [9].
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