Abstract
Daclatasvir and sofosbuvir in pure and medicinal dosage forms can be determined quickly and easily using a reversed-phase high-performance liquid chromatography (RP-HPLC) technique. An accurate, reproducible method was developed and validated. The mobile phase contained a mixture of 90% methanol:10% water (0.05% OPA). UV estimation was done with a flow rate of 0.7 mL/min and wavelength of 275 nm. The temperature was 30°C. Sofosbuvir stays in the body for 3.361 minutes and daclatasvir for 5.745 minutes. A quantitative study of commercial dosage forms went well with this method, which was made and tested. Sofosbuvir and daclatasvir each had a %RSD of 0.43 and 0.28, respectively. Recoveries were 97.85 and 98.52% for sofosbuvir and daclatasvir, respectively. Three methods were checked for precision, accuracy, linearity, selectivity, specificity, limit of detection (LoD), limit of quantitation (LoQ), robustness, and ruggedness according to rules of the International Council for Harmonisation (ICH).
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
